1、蛋白蛋 白 质 的分结 分 子 结 构The Molecular Structure of Protein 第 二 节一级结构一级结构二级结构二级结构三级结构三级结构四级结构四级结构空间结构空间结构是不是所有的蛋白质都有一 、二、蛋白三、分结四级结构呢?是蛋白不是所有的蛋白质都有一、二、分结三 、蛋白四级结构呢
?蛋白质的分结一级结构指多肽链中氨基酸的排列顺序 。一、蛋白蛋白质的分结一级结构一、蛋白质的蛋白一级结构 (primary structure)主要的化学键肽键,有些蛋白质还包括二硫键
。分结 Ser-Val-Tyr-Cys; SVYC牛胰岛素的蛋白一级结构牛胰岛素的一级结构Frederick Sanger is an English bioch
2、emist and a two-time Nobel laureate in chemistry,分结 the only person to have been so. In 1958, he was awarded a Nobel prize in chemistry “for his work on the structure of proteins, especially that of insulin”. In 1980,Walter Gilbert and Sanger shared half of the chemistry prize “for their contributions
3、 concerning the determination of base sequences in nucleic acids”. The蛋白 other half was awarded to Paul Berg “for his fundamental studies of the biochemistry of nucleic acids, with particular regard to recombinant-DNA”. 蛋白质的一级结构研究蛋白质的一级结构研究 蛋白质的氨基酸组成测定:酸水解;碱水解。断裂二硫键:过甲酸氧化法;巯基化合物还原法。末端氨基酸残基测定
:N末端
:PITC法
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、形式 -螺旋 ( -helix ) -折叠 ( -pleated sheet ) -转角 ( -turn ) 无规卷曲 ( random coil ) Linus Carl PaulingLinus Pauling is one of only two people awarded two Nobel Prizes in different fields (the Chemistry and Peace prizes), and the only person a w a r d e d t w o unshared prizes. - -螺旋螺旋 - -螺旋螺旋3.6,0.54 ,0.15平行